Journal: Cellular and Molecular Life Sciences: CMLS
Article Title: Enhanced complement activation and MAC formation accelerates severe COVID-19
doi: 10.1007/s00018-024-05430-w
Figure Lengend Snippet: Deficiency of C7 protects against severe COVID-19. A – I Mixed sex C7 +/+ ( n = 14, 10 males, 4 females) and C7 −/− mice ( n =11, 7 males, 4 females) aged 15–17 weeks were infected with an infectious dose of MA30 SARS-CoV-2 (2.5×10 4 TCID 50 ) and euthanized at 4 and 7 DPI. A Percent body weight changes as assessed by Two-way ANOVA. B viral load after infection as measured by qPCR. C Hemolytic assay showing complement activity as measured by serial dilution of the infected sera as a source of the complement to lyse the anti-body sensitized sheep erythrocytes. D - E C3a ( D ) and C5a ( E ) measurements of the sera from the infected mice by ELISA with significant differences as assessed by One-way ANOVA with Dunnet’s ( D ) or Tukey’s ( E ) multiple comparisons. F - G Representative images of pulmonary edema at 4 DPI (F) and quantification ( G ) analyzed by Student t -test. H - I vWF ( H ) and VEGF ( I ) serum levels (ng/mL) at 4 and 7 DPI. Overall signficiance assessed by One-way ANOVA and indicated by horizontal bar with individual comparisons assessed by two-tailed Student t -test. * p < 0.05, ** p < 0.01, **** p < 0.0001
Article Snippet: C5a ELISA 1:50 dilution (R&D systems, DY2150), C3a ELISA 1:10 dilution (R&D systems, NBP2-70,037), VEGF ELISA 1:20 (R&D systems, MMV00-1), and vWF ELISA 1:5 dilution (LSBio, LS-F22891).
Techniques: Infection, Hemolytic Assay, Activity Assay, Serial Dilution, Enzyme-linked Immunosorbent Assay, Two Tailed Test
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